Investigation of candidate genes and mechanisms underlying obesity associated type 2 diabetes mellitus using bioinformatics analysis and screening of small drug molecules

Abstract Background Obesity associated type 2 diabetes mellitus is a metabolic disorder ; however, the etiology of obesity remains largely unknown. There an urgent need to further broaden understanding molecular mechanism in mellitus. Methods To screen differentially expressed genes (DEGs) that might play essential roles mellitus, publicly available expression profiling by high throughput sequencing data (GSE143319) was downloaded and screened for DEGs. Then, Gene Ontology (GO) REACTOME pathway enrichment analysis were performed. The protein - interaction network, miRNA target regulatory network TF-target gene constructed analyzed identification hub genes. validated receiver operating characteristic (ROC) curve RT- PCR analysis. Finally, docking study performed on over proteins predict small drug molecules. Results A total 820 DEGs identified between healthy obese metabolically unhealthy obese, among 409 up regulated 411 down GO results showed these significantly enriched ion transmembrane transport, intrinsic component plasma membrane, transferase activity, transferring phosphorus-containing groups, cell adhesion, integral membrane signaling receptor binding, whereas, integration energy metabolism extracellular matrix organization. CEBPD, TP73, ESR2, TAB1, MAP 3K5, FN1, UBD, RUNX1, PIK3R2 TNF, which role screened. Conclusions present could deepen be useful developing therapeutic targets